Plasmid_Backbone
Part:BBa_K4961000:Design
Designed by: Liu Yue Group: iGEM23_YangzhouNOFLS (2023-10-06)
PEE12.4
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 689
Illegal SpeI site found at 5980
Illegal PstI site found at 258
Illegal PstI site found at 3692
Illegal PstI site found at 5421
Illegal PstI site found at 7506 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NheI site found at 3334
Illegal SpeI site found at 5980
Illegal PstI site found at 258
Illegal PstI site found at 3692
Illegal PstI site found at 5421
Illegal PstI site found at 7506
Illegal NotI site found at 247 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 689
Illegal SpeI site found at 5980
Illegal PstI site found at 258
Illegal PstI site found at 3692
Illegal PstI site found at 5421
Illegal PstI site found at 7506 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 689
Illegal SpeI site found at 5980
Illegal PstI site found at 258
Illegal PstI site found at 3692
Illegal PstI site found at 5421
Illegal PstI site found at 7506
Illegal NgoMIV site found at 474
Illegal NgoMIV site found at 721
Illegal AgeI site found at 4211 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 6973
Illegal BsaI.rc site found at 1898
Illegal BsaI.rc site found at 3697
Illegal SapI site found at 7092
Design Notes
No
Source
Commercial plasmids produced by biological companies