Plasmid_Backbone

Part:BBa_K4961000:Design

Designed by: Liu Yue   Group: iGEM23_YangzhouNOFLS   (2023-10-06)


PEE12.4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 689
    Illegal SpeI site found at 5980
    Illegal PstI site found at 258
    Illegal PstI site found at 3692
    Illegal PstI site found at 5421
    Illegal PstI site found at 7506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 3334
    Illegal SpeI site found at 5980
    Illegal PstI site found at 258
    Illegal PstI site found at 3692
    Illegal PstI site found at 5421
    Illegal PstI site found at 7506
    Illegal NotI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 689
    Illegal SpeI site found at 5980
    Illegal PstI site found at 258
    Illegal PstI site found at 3692
    Illegal PstI site found at 5421
    Illegal PstI site found at 7506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 689
    Illegal SpeI site found at 5980
    Illegal PstI site found at 258
    Illegal PstI site found at 3692
    Illegal PstI site found at 5421
    Illegal PstI site found at 7506
    Illegal NgoMIV site found at 474
    Illegal NgoMIV site found at 721
    Illegal AgeI site found at 4211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 6973
    Illegal BsaI.rc site found at 1898
    Illegal BsaI.rc site found at 3697
    Illegal SapI site found at 7092


Design Notes

No


Source

Commercial plasmids produced by biological companies

References